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For email login, not currently. But if you use GitHub login or SAML login you can require 2FA via those identity providers. 

I was trying to use bitbucket membership requirements, but it doesn't seem to actually use bitbucket to login

Yeah - the "bitbucket" login is via Atlassian identity, not BitBucket specifically.  If you have <https://support.atlassian.com/security-and-access-policies/docs/enforce-two-step-verification/> configured on your Atlassian account then access to Pulumi should be gated through that 2FA requirement.

<@UB3BGTV63> It seems like that simply enforces that they are members, not that they actually authenticate via atlassian

e.g. I can still log into pulumi using my pulumi username / password even though it requires bitbucket membership

Debating if we want to just migrate to a S3 backend to enforce 2FA to the data. It seems like that's the only option?